Leader

Lilla Turiák

Research

  • Glycomic characterization of tumor-derived extracellular vesicles:
    The research aims to develop methods for characterizing N-glycopeptides and glycosaminoglycans (GAGs) in extracellular vesicles (EVs) isolated from lung cancer cell lines. The long term goal is to identify predictive glycan markers in circulating tumor-derived extracellular vesicles predicting resistance against immune checkpoint inhibitor therapy in non-small cell lung cancer.
  • Identification of predictive biomarkers in lung cancer:
    The aim of our research is to identify key proteins and glycans of biochemical processes leading to different types of lung cancer mainly using mass spectrometry-based proteomics and glycomics. The study focuses on analysing formalin-fixed paraffin embedded tissue sections of lung cancer patients and applying sample preparation protocols developed by the group. A further aim is to identify marker proteins, protein pathways, and glycans that can predict the possible therapeutic effectiveness of a given biological therapy for a lung cancer patient more effectively.
  • Method developments for mass spectrometry based structural characterization of glycosaminoglycans:
    The aim is to develop sensitive HPLC-MS methods for characterizing glycosaminoglycan disaccharides derived from biological samples.

Equipment

Bruker Maxis II ETD mass spectrometer coupled to Waters nanoAcquity UPLC (proteomics, glycoproteomics)
Waters Q-TOF Premier mass spectrometer coupled to Waters nanoAcquity UPLC (GAG disaccharide analysis)

Cooperation

The Glycan Biomarker Research Group is in close cooperation with the following institutions:

HUN-REN TTK Institute of Organic Chemistry

HUN-REN TTK Institute of Molecular Life Sciences

HUN-REN TTK Institute of Materials and Environmental Sciences

Semmelweis University

National Korányi Institute of Pulmonology

University of Groningen

University of Coppenhagen

Recent publications

Distinct proteomic and glycosylation signatures differentiate A549 tumor and BEAS-2B non-tumor cell line derived small extracellular vesicles

Balbisi M, Langó T, Horváth VN, Pál D, Schlosser G, Kecskeméti G, Szabó Z, Ilyés K, Nagy N, Tóth O, Zheng J, Lageveen-Kammeijer GSM, Visnovitz T, Varga Z, Vértessy BG, Turiák L.

Mol Cell Proteomics. 2026 Feb 3.

doi: 10.1016/j.mcpro.2026.101524

Capillary electrophoresis in glycosaminoglycan analysis

Dessidianti R, Grabarics M, Virág D, Lageveen-Kammeijer GSM, Szökő É, Turiák L.

Talanta. 2026 Jan 18.

doi: 10.1016/j.talanta.2026.129429

Unveiling unique protein and phosphorylation signatures in lung adenocarcinomas with and without ALK, EGFR, and KRAS genetic alterations

Bugyi F, Balbisi M, Sugár S, Váncza L, Regős E, Kovalszky I, Laczó I, Harkó T, Kecskeméti G, Szabó Z, Moldvay J, Drahos L, Turiák L.

Mol Oncol. 2025 Jul 7.

doi: 10.1002/1878-0261.70091

Analysis and characterization of chondroitin/dermatan sulfate composition of lung adenocarcinoma tissues with different types of genetic alterations in ALK, EGFR and KRAS oncogenes

Pál D, Bugyi F, Virág D, Szabó D, Schlosser G, Kovalszky I, Harkó T, Moldvay J, Turiák L.

Carbohydrate Polymer Technologies and Applications. 2025 May 3.

doi: 10.1016/j.carpta.2025.100826

Glycoproteomics Analysis of Triple Wild-Type Lung Adenocarcinoma Tissue Samples.

Sugár S, Molnár B, Bugyi F, Kecskeméti G, Szabó Z, Laczó I, Harkó T, Moldvay J, Turiák L.

Journal of Proteome Research. 2025 April 2.

doi: 10.1021/acs.jproteome.4c01063

Investigation of sample handling steps for accurate heparan sulphate disaccharide analysis using HPLC-MS.

Pál D, Tóth G, Turiák L.

Electrophoresis. 2024 Aug 21.

doi: 10.1002/elps.202400091.

Protein glycosylation in lung cancer from a mass spectrometry perspective.

Balbisi M, Sugár S, Turiák L.

Mass Spectrom Rev. 2024 Apr 4.

doi: 10.1002/mas.21882.

Spatially Resolved Proteomic and Transcriptomic Profiling of Anaplastic Lymphoma Kinase-Rearranged Pulmonary Adenocarcinomas Reveals Key Players in Inter- and Intratumoral Heterogeneity.

Szeitz B, Glasz T, Herold Z, Tóth G, Balbisi M, Fillinger J, Horváth S, Mohácsi R, Kwon HJ, Moldvay J, Turiák L, Szász AM.

Int J Mol Sci. 2023 Jul 12;24(14):11369.

doi: 10.3390/ijms241411369.

Compositional Analysis of Glycosaminoglycans in Different Lung Cancer Types-A Pilot Study.

Pál D, Tóth G, Sugár S, Fügedi KD, Szabó D, Kovalszky I, Papp D, Schlosser G, Tóth C, Tornóczky T, Drahos L, Turiák L.

Int J Mol Sci. 2023 Apr 11;24(8):7050.

doi: 10.3390/ijms24087050.

Inter- and intratumoral proteomics and glycosaminoglycan characterization of ALK rearranged lung adenocarcinoma tissues: a pilot study.

Balbisi M, Sugár S, Schlosser G, Szeitz B, Fillinger J, Moldvay J, Drahos L, Szász AM, Tóth G, Turiák L.

Sci Rep. 2023 Apr 17;13(1):6268.

doi: 10.1038/s41598-023-33435-1.

HPLC-MS Characterization of Tissue-Derived Heparan Sulfate and Chondroitin Sulfate.

Tóth G, Turiák L.

Methods Mol Biol. 2023;2619:71-90.

doi: 10.1007/978-1-0716-2946-8_6.

Comparison of solid-phase extraction methods for efficient purification of phosphopeptides with low sample amounts.

Bugyi F, Tóth G, Kovács KB, Drahos L, Turiák L.

J Chromatogr A. 2022 Dec 6;1685:463597.

doi: 10.1016/j.chroma.2022.463597.

Optimized Sample Preparation and Microscale Separation Methods for High-Sensitivity Analysis of Hydrophilic Peptides.

Tóth G, Sugár S, Balbisi M, Molnár BA, Bugyi F, Fügedi KD, Drahos L, Turiák L.

Molecules. 2022 Oct 6;27(19):6645.

doi: 10.3390/molecules27196645.

Glycosaminoglycan Analysis of FFPE Tissues from Prostate Cancer and Benign Prostate Hyperplasia Patients Reveals Altered Regulatory Functions and Independent Markers for Survival.

Tóth G, Sugár S, Pál D, Fügedi KD, Drahos L, Schlosser G, Oláh C, Reis H, Kovalszky I, Szarvas T, Turiák L.

Cancers (Basel). 2022 Oct 5;14(19):4867.

doi: 10.3390/cancers14194867.

Proteomic Analysis of Lung Cancer Types-A Pilot Study.

Sugár S, Bugyi F, Tóth G, Pápay J, Kovalszky I, Tornóczky T, Drahos L, Turiák L.

Cancers (Basel). 2022 May 26;14(11):2629.

doi: 10.3390/cancers14112629.

Expression of glycosaminoglycans in cirrhotic liver and hepatocellular carcinoma-a pilot study including etiology.

Tóth G, Pál D, Sugár S, Kovalszky I, Dezső K, Schlosser G, Drahos L, Turiák L.Anal Bioanal Chem. 2022 May;414(13):3837-3846.

doi: 10.1007/s00216-022-04025-3.

Alterations in protein expression and site-specific N-glycosylation of prostate cancer tissues.

Sugár S, Tóth G, Bugyi F, Vékey K, Karászi K, Drahos L, Turiák L.

Sci Rep. 2021 Aug 5;11(1):15886.

doi: 10.1038/s41598-021-95417-5.

Stability and recovery issues concerning chondroitin sulfate disaccharide analysis.

Tóth G, Pál D, Vékey K, Drahos L, Turiák L.

Anal Bioanal Chem. 2021 Mar;413(7):1779-1785.

doi: 10.1007/s00216-021-03152-7.

News

We participated in the MassSpec Forum held in Vienna on 18-19 February with four posters (Mirjam Balbisi, Boglárka Cziráki, Rachma Dessidianti, Alexandra Molnár).

The ELTE Institute of Chemistry TDK conference was held on 6 February, where Boglárka Cziráki took third place in the Organic Chemistry section. Congratulations!

Júlia Németh joined our group in February. We wish her all the best!

Domonkos Pál successfully defended his PhD dissertation on 8 December. Congratulations!

As part of the EMBO Advanced Collaboration Grant, Mirjam Balbisi spent three months at the University of Groningen, where she worked on CE and LC-MS analysis of HS disaccharides. We would like to thank Guinevere Lageveen-Kammeijer’s team for their cooperation!

On 19 November, the TDK conference was held at BME, where Virág Nikolett Horváth won 2nd prize in the Analytical and Computational Chemistry section. Congratulations!

Between 14 and 17 October, we successfully organized the CEEPC conference in Budapest in collaboration with the MS Proteomics Research Group. Our group presented one oral presentation (Lilla Turiák) and seven poster presentations (Boglárka Cziráki, Rachma Dessidianti, Virág Nikolett Horváth, Krisztián Márk Karvaly, Viktória Kiss, Alexandra Molnár, Domonkos Pál) at the conference. Krisztián Márk Karvaly and Alexandra Molnár won poster awards. Congratulations!

Members