Leader
Research
- Glycomic characterization of tumor-derived extracellular vesicles (Hungarian Academy of Sciences Lendület Program, Lendület LP2023-19/2023):
The research aims to develop methods for characterizing N-glycopeptides and glycosaminoglycans (GAGs) in extracellular vesicles (EVs) isolated from lung cancer cell lines. The long term goal is to identify predictive glycan markers in circulating tumor-derived extracellular vesicles predicting resistance against immune checkpoint inhibitor therapy in non-small cell lung cancer. - Method developments for mass spectrometry based structural characterization of glycosaminoglycans:
The aim is to develop sensitive HPLC-MS methods for characterizing glycosaminoglycan disaccharides derived from biological samples. - Glycomic and glycoproteomic characterization of cell lines: HORIZON-WIDERA-2025-03 Project: 101270048 — IMProGlycAdd (Hop-on Facility to EIC Pathfinder Open scheme 101186774 – IMProGlyco, https://improglyco.science/).
The IMProGlyco team is developing an exciting new strategy to engineer glycans in mammalian cells. This approach will be used to tailor the production of individual proteins and cell lines, changing the pattern of observed glycoforms to modulate their activity and hence pharmacological properties.
The IMProGlyco project is a collaboration between the following institutions: University of Leeds, University of York, BOKU, Oslo Metropolitan University, Oslo University Hospital, GlycoSeLect, the Centre for Process Innovation, and HUN-REN TTK. - Effect of plant-derived extracellular vesicles on the proteome of human and plant cells (MTA Bilateral project mobility 2025, NKM2025-67. Partner institute: Institute of Biosciences and BioResources, National Research Council of Italy, Naples, Italy. Project leader at partner institute: Pócsfalvi Gabriella):
Plant-derived nanovesicles (PDNVs) are promising biotechnological tools. These naturally occurring, biocompatible nanostructures offer a unique opportunity for the delivery of bioactive molecules within various organisms. The aim of this project is to investigate PDNVs as delivery vehicles for targeted therapies in human and plant cells.
Equipment
Bruker Maxis II ETD mass spectrometer coupled to Waters nanoAcquity UPLC (proteomics, glycoproteomics)
Waters Q-TOF Premier mass spectrometer coupled to Waters nanoAcquity UPLC (GAG disaccharide analysis)
Cooperation
The Glycan Biomarker Research Group is in close cooperation with the following institutions:
HUN-REN TTK Institute of Organic Chemistry
HUN-REN TTK Institute of Molecular Life Sciences
HUN-REN TTK Institute of Materials and Environmental Sciences
Semmelweis University
National Korányi Institute of Pulmonology
University of Groningen
University of Coppenhagen
Recent publications
Balbisi M, Langó T, Horváth VN, Pál D, Schlosser G, Kecskeméti G, Szabó Z, Ilyés K, Nagy N, Tóth O, Zheng J, Lageveen-Kammeijer GSM, Visnovitz T, Varga Z, Vértessy BG, Turiák L.
Mol Cell Proteomics. 2026 Feb 3.
doi: 10.1016/j.mcpro.2026.101524
Capillary electrophoresis in glycosaminoglycan analysis
Dessidianti R, Grabarics M, Virág D, Lageveen-Kammeijer GSM, Szökő É, Turiák L.
Talanta. 2026 Jan 18.
doi: 10.1016/j.talanta.2026.129429
Bugyi F, Balbisi M, Sugár S, Váncza L, Regős E, Kovalszky I, Laczó I, Harkó T, Kecskeméti G, Szabó Z, Moldvay J, Drahos L, Turiák L.
Mol Oncol. 2025 Jul 7.
doi: 10.1002/1878-0261.70091
Pál D, Bugyi F, Virág D, Szabó D, Schlosser G, Kovalszky I, Harkó T, Moldvay J, Turiák L.
Carbohydrate Polymer Technologies and Applications. 2025 May 3.
doi: 10.1016/j.carpta.2025.100826
Glycoproteomics Analysis of Triple Wild-Type Lung Adenocarcinoma Tissue Samples.
Sugár S, Molnár B, Bugyi F, Kecskeméti G, Szabó Z, Laczó I, Harkó T, Moldvay J, Turiák L.
Journal of Proteome Research. 2025 April 2.
doi: 10.1021/acs.jproteome.4c01063
Pál D, Tóth G, Turiák L.
Electrophoresis. 2024 Aug 21.
doi: 10.1002/elps.202400091.
Protein glycosylation in lung cancer from a mass spectrometry perspective.
Balbisi M, Sugár S, Turiák L.
Mass Spectrom Rev. 2024 Apr 4.
doi: 10.1002/mas.21882.
Szeitz B, Glasz T, Herold Z, Tóth G, Balbisi M, Fillinger J, Horváth S, Mohácsi R, Kwon HJ, Moldvay J, Turiák L, Szász AM.
Int J Mol Sci. 2023 Jul 12;24(14):11369.
doi: 10.3390/ijms241411369.
Compositional Analysis of Glycosaminoglycans in Different Lung Cancer Types-A Pilot Study.
Pál D, Tóth G, Sugár S, Fügedi KD, Szabó D, Kovalszky I, Papp D, Schlosser G, Tóth C, Tornóczky T, Drahos L, Turiák L.
Int J Mol Sci. 2023 Apr 11;24(8):7050.
doi: 10.3390/ijms24087050.
Balbisi M, Sugár S, Schlosser G, Szeitz B, Fillinger J, Moldvay J, Drahos L, Szász AM, Tóth G, Turiák L.
Sci Rep. 2023 Apr 17;13(1):6268.
doi: 10.1038/s41598-023-33435-1.
HPLC-MS Characterization of Tissue-Derived Heparan Sulfate and Chondroitin Sulfate.
Tóth G, Turiák L.
Methods Mol Biol. 2023;2619:71-90.
doi: 10.1007/978-1-0716-2946-8_6.
Bugyi F, Tóth G, Kovács KB, Drahos L, Turiák L.
J Chromatogr A. 2022 Dec 6;1685:463597.
doi: 10.1016/j.chroma.2022.463597.
Tóth G, Sugár S, Balbisi M, Molnár BA, Bugyi F, Fügedi KD, Drahos L, Turiák L.
Molecules. 2022 Oct 6;27(19):6645.
doi: 10.3390/molecules27196645.
Tóth G, Sugár S, Pál D, Fügedi KD, Drahos L, Schlosser G, Oláh C, Reis H, Kovalszky I, Szarvas T, Turiák L.
Cancers (Basel). 2022 Oct 5;14(19):4867.
doi: 10.3390/cancers14194867.
Proteomic Analysis of Lung Cancer Types-A Pilot Study.
Sugár S, Bugyi F, Tóth G, Pápay J, Kovalszky I, Tornóczky T, Drahos L, Turiák L.
Cancers (Basel). 2022 May 26;14(11):2629.
doi: 10.3390/cancers14112629.
Tóth G, Pál D, Sugár S, Kovalszky I, Dezső K, Schlosser G, Drahos L, Turiák L.Anal Bioanal Chem. 2022 May;414(13):3837-3846.
doi: 10.1007/s00216-022-04025-3.
Alterations in protein expression and site-specific N-glycosylation of prostate cancer tissues.
Sugár S, Tóth G, Bugyi F, Vékey K, Karászi K, Drahos L, Turiák L.
Sci Rep. 2021 Aug 5;11(1):15886.
doi: 10.1038/s41598-021-95417-5.
Stability and recovery issues concerning chondroitin sulfate disaccharide analysis.
Tóth G, Pál D, Vékey K, Drahos L, Turiák L.
Anal Bioanal Chem. 2021 Mar;413(7):1779-1785.
doi: 10.1007/s00216-021-03152-7.
News
We have successfully joined the IMProGlyco Horizon project as a widening partner. The project focuses on research into the targeted modification of glycans in mammalian cells.
We participated in the MassSpec Forum held in Vienna on 18-19 February with four posters (Mirjam Balbisi, Boglárka Cziráki, Rachma Dessidianti, Alexandra Molnár).
The ELTE Institute of Chemistry TDK conference was held on 6 February, where Boglárka Cziráki took third place in the Organic Chemistry section. Congratulations!
Júlia Németh joined our group in February. We wish her all the best!
Domonkos Pál successfully defended his PhD dissertation on 8 December. Congratulations!
As part of the EMBO Advanced Collaboration Grant, Mirjam Balbisi spent three months at the University of Groningen, where she worked on CE and LC-MS analysis of HS disaccharides. We would like to thank Guinevere Lageveen-Kammeijer’s team for their cooperation!
On 19 November, the TDK conference was held at BME, where Virág Nikolett Horváth won 2nd prize in the Analytical and Computational Chemistry section. Congratulations!
Between 14 and 17 October, we successfully organized the CEEPC conference in Budapest in collaboration with the MS Proteomics Research Group. Our group presented one oral presentation (Lilla Turiák) and seven poster presentations (Boglárka Cziráki, Rachma Dessidianti, Virág Nikolett Horváth, Krisztián Márk Karvaly, Viktória Kiss, Alexandra Molnár, Domonkos Pál) at the conference. Krisztián Márk Karvaly and Alexandra Molnár won poster awards. Congratulations!
Alumni
- Márkó Grabarics – postdoctoral researcher
- Gábor Tóth – PhD
- Simon Sugár – PhD
- Fanni Bugyi – PhD
- Domonkos Pál – PhD
- Kata Dorina Fügedi – MSc
- Balázs Molnár – BSc, MSc
- Virág Nikolett Horváth – MSc
- Viktória Kiss – BSc